Research Output

Anandamide induces cardiorespiratory reflexes via vasosensory nerves in the anaesthetized rat.

  We tested the hypothesis that sensory nerves innervating blood vessels play a role in the local and systemic regulation of the cardiovascular and respiratory (CVR) systems. We measured CVR reflexes evoked by administration of anandamide (86 – 863 nmoles) and capsaicin (0.3 – 10 nmoles) into the hindlimb vasculature of anaesthetized rats.
Anandamide and capsaicin each caused a rapid dose-dependent reflex fall in blood pressure and an increase in ventilation when injected intra-arterially into the hindlimb.
Action of both agonists at the vanilloid receptor (VR1) on perivascular sensory nerves was investigated using capsazepine (1 mg kg-1 i.a.) a competitive VR1 antagonist, ruthenium red (1 mg kg-1 i.a.), a non-competitive antagonist at VR1, or a desensitizing dose of capsaicin (200 nmoles i.a.). The cannabinoid receptor antagonist SR141716 (1 mg kg-1 i.a.) was used to determine agonist activity at the CB1 receptor.
Capsazepine, ruthenium red, or acute VR1 desensitization by capsaicin-pretreatment, markedly attenuated the reflex CVR responses evoked by anandamide and capsaicin (P

  • Type:

    Article

  • Date:

    01 October 2001

  • Publication Status:

    Published

  • Publisher

    Wiley-Blackwell

  • DOI:

    10.1038/sj.bjp.0704296

  • ISSN:

    0007-1188

Citation

Smith, P. & McQueen, D. S. (2001). Anandamide induces cardiorespiratory reflexes via vasosensory nerves in the anaesthetized rat. British Journal of Pharmacology. 134, 655-663. doi:10.1038/sj.bjp.0704296. ISSN 0007-1188

Keywords

Anandamide; Capsaicin; Effects; Sensory nerves; Cardiovascular-respiratory reflexes; Vanilloid receptor; Rats;

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