Research Output

Effect ofNippostrongylus brasiliensisL3 ES on inflammatory mediatorgene transcription in lipopolysaccharide lung inflammation

  The anti-inflammatory properties of parasitic helminths have
been largely linked to their excretory–secretory (ES) products.
Some studies have noted a lack of TNF-α production and limited
recruitment of neutrophils into the lungs after Nippostrongylus
brasiliensis infection. We previously reported that instillation
of ES from L3 larvae of N. brasiliensis to the lungs could
inhibit the recruitment of neutrophils on a background of
LPS-induced inflammation. A similar reduction in neutrophil
recruitment was observed in this study. This reduction was
associated with the significant inhibition in gene transcription
of the adhesion molecule, ICAM-1, and the chemokine, MIP-
2 in bronchoalveolar lavage (BAL) cells. The LPS-stimulated
gene transcription of the pro-inflammatory cytokines TNF-
α and IL-1β was also significantly reduced by L3 ES. Inducible
nitric oxide synthase (iNOS) is normally elevated in classically
activated macrophages, however, in this case gene transcription
of iNOS was inhibited by L3 ES and may suggest a phenotype
change to anti-inflammatory. The general inhibition of
pro-inflammatory mediators observed in this study suggests
that infective stage L3 larvae excrete and/or secrete inhibitory
products capable of modifying the normally potent LPS
inflammatory response.

  • Type:

    Article

  • Date:

    13 October 2008

  • Publication Status:

    Published

  • Publisher

    Blackwell Syngery

  • DOI:

    10.1111/j.1365-3024.2008.001073.x

  • ISSN:

    0141-9838

  • Library of Congress:

    QP Physiology

  • Dewey Decimal Classification:

    571 Physiology & related subjects

  • Funders:

    Edinburgh Napier Funded

Citation

Zhao, M., Brown, D. M., MacCallum, J. & Proudfoot, L. (2008). Effect ofNippostrongylus brasiliensisL3 ES on inflammatory mediatorgene transcription in lipopolysaccharide lung inflammation. Parasite Immunology. 31, 50-56. doi:10.1111/j.1365-3024.2008.001073.x. ISSN 0141-9838

Authors

Keywords

anti-inflammatory, bronchoalveolar lavage (BAL), chemokines, cytokines, lung, Nippostrongylus brasiliensis

Monthly Views:

Available Documents