Research Output

Improved protection against avian influenza H5N1 virus by a single microneedle skin vaccination with virus-like particles.

  To develop a more effective vaccination method against H5N1 virus, we investigated the immunogenicity and protective efficacy after skin vaccination using microneedles coated with influenza virus-like particles containing hemagglutinin derived from A/Vietnam/1203/04 H5N1 virus (H5 VLPs). A single microneedle vaccination of mice with H5 VLPs induced increased levels of antibodies and provided complete protection against lethal challenge without apparent disease symptoms. In contrast, intramuscular injection with the same vaccine dose showed low levels of antibodies and provided only partial protection accompanied by severe body weight loss. Post-challenge analysis suggested that improved protection was associated with lower lung viral titers and enhanced generation of recall antibody secreting cells by microneedle vaccination. Thus, this study provides evidence that skin delivery of H5 VLP vaccines using microneedles designed for self-administration induces improved protection compared to conventional intramuscular immunization.

  • Type:

    Article

  • Date:

    31 October 2010

  • Publication Status:

    Published

  • Publisher

    Elsevier

  • DOI:

    10.1016/j.antiviral.2010.09.001

  • Library of Congress:

    RF Otorhinolaryngology

  • Dewey Decimal Classification:

    616.2 Respiratory diseases

Citation

Song, J., Kim, Y., Barlow, P. G., Hossain, M. J., Park, K., Donis, R. O., …Kang, S. (2010). Improved protection against avian influenza H5N1 virus by a single microneedle skin vaccination with virus-like particles. Antiviral research. 88, 244-247. doi:10.1016/j.antiviral.2010.09.001. ISSN 0166-3542

Authors

Keywords

H5N1; Pandemic vaccine; Single dose; Skin vaccination; Microneedles

Available Documents