Research Output

Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas.

  Ovulation is believed to contribute to the development of ovarian cancers that derive from the ovarian surface epithelium (OSE). The process of ovulation is synonymous with inflammation and inflammatory cytokines such as interleukin-1α (IL-1α) have recently been shown to induce both inflammatory and anti-inflammatory responses in human OSE (HOSE) cells. In this study we directly compared levels of IL-1α-induced gene expression by analysing the levels of I I /?-hydroxysteroid dehydrogenase (11βHSD) types I (I IβHSD-1) and 2 (IIβHSD-2), cyclooxygenase-2 (COX-2), IL-I receptor (IL-IR) and glucocorticoid receptor α (GRα) mRNA between normal HOSE cells and cell lines derived from poorly differentiated (SKOV-3, BG-1, PEO-4) and well-differentiated (PEO-14) ovarian adenocarcinoma. In HOSE cell cultures, and to a lesser extent PEO-14 cells, the basal mRNA levels of COX-2 and I 1βHSD-1 were relatively high and further shown to be induced in response to IL-1α (for HOSE cells; >20-fold, P3fold, P

  • Type:

    Article

  • Date:

    01 May 2005

  • Publication Status:

    Published

  • Publisher

    Nature Publishing Group

  • DOI:

    10.1038/sj.bjc.6602568

  • ISSN:

    0007-0920

Citation

Gubbay, O., Guo, W., Rae, M. T., Niven, D., Langdon, S. P. & Hillier, S. G. (2005). Inflammation-associated gene expression is altered between normal human ovarian surface epithelial cells and cell lines derived from ovarian adenocarcinomas. British Journal of Cancer. 92, 1927-33. doi:10.1038/sj.bjc.6602568. ISSN 0007-0920

Authors

Keywords

Malignant tumor ; Female genital diseases ; Female genital system ; Ovarian diseases ; Ovary adenocarcinoma ; Cancerology ; Epithelium ; Established cell line ; Epithelial cell ; Cell surface ; Human ; Gene expression ; Inflammation ; Ovary cancer ;

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