Senior Lecturer, Teaching Fellow, Head of Life Sciences
Simpson, R. J., Graham, S. M., Florida-James, G. D., Connaboy, C., Clement, R. & Jackson, A. S. (2010). Perceived exertion and heart rate models for estimating metabolic workload in elite British soldiers performing a backpack load-carriage task. Applied physiology, nutrition, and metabolism = Physiologie appliquée, nutrition et métabolisme. 35(5), 650-656. doi:10.1139/h10-053. ISSN 1715-5320
Identifying field measures to estimate backpack load-carriage work intensity in elite soldiers is of interest to the military. This study developed rating of perceived exertio...
Fox, S., Wilkinson, T. S., Wheatley, P. S., Xiao, B., Morris, R. E., Sutherland, A., …Rossi, A. G. (2010). NO-loaded Zn2+-exchanged zeolite materials: A potential bifunctional anti-bacterial strategy. Acta biomaterialia. 6, 1515-1521. doi:10.1016/j.actbio.2009.10.038. ISSN 1742-7061
Nitric oxide (NO) is important for the regulation of a number of diverse biological processes, including vascular tone, neurotransmission, inflammatory cell responsiveness, de...
Fraser, J. A., Madhumalar, A., Blackburn, E., Bramham, J., Walkinshaw, M. D., Verma, C. & Hupp, T. R. (2009). A Novel p53 Phosphorylation Site within the MDM2 Ubiquitination Signal: II. A MODEL IN WHICH PHOSPHORYLATION AT SER269 INDUCES A MUTANT CONFORMATION TO p53. Journal of Biological Chemistry. 285, 37773-37786. doi:10.1074/jbc.M110.143107. ISSN 0021-9258
The p53 DNA-binding domain harbors a conformationally flexible multiprotein binding site that regulates p53 ubiquitination. A novel phosphorylation site exists within this reg...
Chief Scientists Office
We have identified the human cathelicidn LL-37 as having potent direct antiviral activity against human rhinovirus (HRV), together with the capacity to alter death pathways in HRV-infected cells, as a...
Crohn's in Childhood Research Association
An investigation of commonly used IBD drugs on autophagy pathway activity and potential therapeutic benefit for treatment of paediatric IBD
Medical Research Council
Risks of developing many adult illnesses are set down as we develop in the womb, hence the environment we experience during fetal life needs to be ‘just right’. Understanding how the fetal environmen...
Dean of Applied Sciences
Vice Chancellor's Research Fellow
Associate Professor of Immunology & Infection / Director of Research, School of Applied Sciences
Lecturer in Biomedical Science
Fraser, J. A., Vojtesek, B. & Hupp, T. R. (2009). A Novel p53 Phosphorylation Site within the MDM2 Ubiquitination Signal: I. PHOSPHORYLATION AT SER269 IN VIVO IS LINKED TO INACTIVATION OF p53 FUNCTION. Journal of Biological Chemistry. 285, 37762-37772. doi:10.1074/jbc.M110.143099. ISSN 0021-9258
p53 is a thermodynamically unstable protein containing a conformationally flexible multiprotein docking site within the DNA-binding domain. A combinatorial peptide chip used t...
Barlow, P. G., Beaumont, P. E., Cosseau, C., Mackellar, A., Wilkinson, T. S., Hancock, R. E. W., …Davidson, D. J. (2009). The human cathelicidin LL-37 preferentially promotes apoptosis of infected airway epithelium. American Journal of Respiratory Cell and Molecular Biology. 43, 692-702. doi:10.1165/rcmb.2009-0250OC. ISSN 1044-1549
Cationic host defense peptides are key, evolutionarily conserved components of the innate immune system. The human cathelicidin LL-37 is an important cationic host defense pep...
Fraser, J. A. (2009). Regulation and Function of the Original p53- Inducible p21 Gene. In p53; Molecular Biology Intelligence Unit, 100-116. Springer Science + Business Media. doi:10.1007/978-1-4419-8231-5_7. ISBN 978-1-4419-8230-8; 978-1-4419-8231-5
P21 is a well known regulator of cell cycle progression through its inhibitory actions on Cyclin dependent kinases, (Cdk)/cyclin complexes, and DNA replication via its binding...
Jacobson, S., Steitz, J., Barlow, P. G., Hossain, M. J., Kim, E., Okada, K., …Gambotto, A. (2009). A candidate H1N1 pandemic influenza vaccine elicits protective Immunity in mice. PLoS ONE. 5, e10492. doi:10.1371/journal.pone.0010492. ISSN 1932-6203
In 2009 a new pandemic disease appeared and spread globally. The recent emergence of the pandemic influenza virus H1N1 first isolated in Mexico and USA raised conce...
Li, H., Barlow, P. G., Bylund, J., Mackellar, A., Bjorstad, A., Conlon, J., …Davidson, D. J. (2008). Secondary necrosis of apoptotic neutrophils induced by the human cathelicidin LL-37 is not proinflammatory to phagocytosing macrophages. Journal of Leukocyte Biology. 86, 891-902. doi:10.1189/jlb.0209050. ISSN 0741-5400
Cathelicidins are CHDP with essential roles in innate host defense but also more recently associated with the pathogenesis of certain chronic diseases. These peptides have mic...
Barlow, P. G., Brown, D. M., Donaldson, K., McCallum, J. & Stone, V. (2008). Reduced alveolar macrophage migration induced by acute ambient particle (PM10) exposure. Cell Biology and Toxicology. 24, 243-252. doi:10.1007/s10565-007-9033-y. ISSN 0742-2091
Increased levels of particulate air pollution (PM10) have been implicated as a causal agent in pulmonary disease exacerbation and increased deaths from respiratory and cardiov...
MacLaine, N. J., Øster, B., Bundgaard, B., Fraser, J. A., Buckner, C., Lazo, P. A., …Hupp, T. R. (2007). A Central Role for CK1 in Catalyzing Phosphorylation of the p53 Transactivation Domain at Serine 20 after HHV-6B Viral Infection. Journal of Biological Chemistry. 283, 28563-28573. doi:10.1074/jbc.M804433200. ISSN 0021-9258
The tumor suppressor protein p53 is activated by distinct cellular stresses including radiation, hypoxia, type I interferon, and DNA/RNA virus infection. The transactivation d...
Craig, A. L., Chrystal, J. A., Fraser, J. A., Sphyris, N., Lin, Y., Harrison, B. J., …Hupp, T. R. (2007). The MDM2 Ubiquitination Signal in the DNA-Binding Domain of p53 Forms a Docking Site for Calcium Calmodulin Kinase Superfamily Members. Molecular and Cellular Biology. 27(9), 3542-3555. doi:10.1128/mcb.01595-06. ISSN 0270-7306
Genetic and biochemical studies have shown that Ser20 phosphorylation in the transactivation domain of p53 mediates p300-catalyzed DNA-dependent p53 acetylation and B-cell tum...
Lin, Y., Stevens, C. & Hupp, T. (2007). Identification of a Dominant Negative Functional Domain on DAPK-1 That Degrades DAPK-1 Protein and Stimulates TNFR-1-mediated Apoptosis. Journal of Biological Chemistry. 282, 16792-16802. doi:10.1074/jbc.M611559200. ISSN 0021-9258
DAPK-1 is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. To define mechanisms of DAPK-1 prot...
Stevens, C., Lin, Y., Sanchez, M., Amin, E., Copson, E., White, H., …Hupp, T. (2007). A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis. Journal of Biological Chemistry. 282, 13791-13803. doi:10.1074/jbc.M605649200. ISSN 0021-9258
p53 is activated genetically by a set of kinases that are components of the calcium calmodulin kinase superfamily, including CHK2, AMP kinase, and DAPK-1. In dissecting the me...
Brown, D., Hutchison, G., Stone, V. & Barlow, P. (2006). Proinflammatory effects of particles on macrophages and epithelial cells. In Particle Toxicology, 183-196. Informa UK Limited. doi:10.1201/9781420003147.ch9. ISBN 978-0-8493-5092-4; 978-1-4200-0314-7
Fraser, J. A. & Hupp, T. R. (2006). Chemical Genetics Approach to Identify Peptide Ligands that Selectively Stimulate DAPK-1 Kinase Activity. Biochemistry. 46(10), 2655-2673. doi:10.1021/bi061562j. ISSN 0006-2960
Dissection of signal transduction pathways has been advanced by classic genetic approaches including targeted gene deletion and siRNA-based inhibition of gene product synthesi...
Society for Reproduction and Fertility
Does the gut microbiome play a role in polycystic ovary syndrome?
The University of Edinburgh
Neuromodulation can improve ovarian dysfunction in polycystic ovary syndrome (PCOS). This is achieved by four mechanisms: i) if neural signals are involved in LH action directly their modulation may ...
Undergraduate vacation scholarship application to study the legacy of prenatal programming of polycystic ovary syndrome on postnatal throid function
Polycystic ovary syndrome (PCOS) is common: more than one in 1 in 15 young women has this condition. There are many associated health problems including abnormal periods, miscarriages, infertility, ex...