Research Output

Carbon nanoparticles inhibit the antimicrobial activities of the human cathelicidin LL-37 through structural alteration

  Host defense peptides, also known as antimicrobial peptides, are key elements of innate host defense. One host defense peptide with well-characterized antimicrobial activity is the human cathelicidin, LL-37. LL-37 has been shown to be upregulated at sites of infection and inflammation and is regarded as one of the primary innate defense molecules against bacterial and viral infection. Human exposure to combustion-derived or engineered nanoparticles is of increasing concern, and the implications of nanomaterial exposure on the human immune response is poorly understood. However, it is widely acknowledged that nanoparticles can interact strongly with several immune proteins of biological significance, with these interactions resulting in structural and functional changes of the proteins involved. This study investigated whether the potent antibacterial and antiviral functions of LL-37 were inhibited by exposure to, and interaction with, carbon nanoparticles, together with characterizing the nature of the interaction. LL-37 was exposed to carbon black nanoparticles in vitro, and the antibacterial and antiviral functions of the peptide were subsequently assessed. We demonstrate a substantial loss of antimicrobial function when the peptide was exposed to low concentrations of nanomaterials, and we further show that the nanomaterial-peptide interaction resulted in a significant change in the structure of the peptide. The human health implications of these findings are significant, as, to our knowledge, this is the first evidence that nanoparticles can alter host defense peptide structure and function, indicating a new role for nanoparticle exposure in increased disease susceptibility.

  • Type:

    Article

  • Date:

    16 August 2017

  • Publication Status:

    Published

  • DOI:

    10.4049/jimmunol.1700706

  • ISSN:

    0022-1767

  • Library of Congress:

    QR180 Immunology

  • Dewey Decimal Classification:

    616 Diseases

  • Funders:

    Edinburgh Napier Funded

Citation

Findlay, F., Pohl, J., Svoboda, P., Shakamuri, P., McLean, K., Inglis, N. F., …Barlow, P. G. (2017). Carbon nanoparticles inhibit the antimicrobial activities of the human cathelicidin LL-37 through structural alteration. Journal of Immunology, 199(7), (2483-2490). doi:10.4049/jimmunol.1700706. ISSN 0022-1767

Authors

Keywords

Carbon nanoparticles, antimicrobial activities, human cathelicidin LL-37,

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