Research Output

Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid.

  Aims:  The aim of the present study was to isolate and characterize a bacterium, strain EN3, capable of using diesel oil as a major carbon and energy source, and to analyse the enhancement of diesel oil degradation by this organism using synthetic mycolic acid (2-hexyl-3-hydroxyldecanoic acid).

Method and Results:  An actinomycete with the ability to degrade diesel oil was isolated from oil contaminated soil and characterized. The strain had phenotypic properties consistent with its classification in the genus Rhodococcus showing a 16S rRNA gene similarity of 99·7% with Rhodococcus baikonurensis DSM 44587T. The ability of the characterized strain to degrade diesel oil at various concentrations (1000, 5000, 10 000 and 20 000 mg l−1) was determined. The effect of synthetic mycolic acid on the biodegradation of diesel oil was investigated at the 20 000 mg l−1 concentration; the surfactant was added to the flask cultures at three different concentrations (10, 50 and 100 mg l−1) and degradation followed over 7 days. Enhanced degradation was found at all three concentrations of the surfactant. In addition, the enhancement of diesel oil degradation by other surfactants was observed.

Conclusions:  The synthetic mycolic acid has potential for the remediation of petroleum-contaminated sites from both an economic and applied perspective as it can stimulate biodegradation at low concentrations.

Significance and Impact of the Study:  This study showed that the synthesized mycolic acid can be used for potential applications in the bioremediation industries, for example, in oil spill clean-up, diesel fuel remediation and biostimulation.

  • Type:

    Article

  • Date:

    17 January 2006

  • Publication Status:

    Published

  • Publisher

    Wiley-Blackwell

  • DOI:

    10.1111/j.1365-2672.2005.02756.x

  • Cross Ref:

    10.1111/j.1365-2672.2005.02756.x

  • ISSN:

    1364-5072

  • Library of Congress:

    QR Microbiology

  • Dewey Decimal Classification:

    579 Microorganisms, fungi & algae

Citation

Lee, M., Kim, M., Singleton, I., Goodfellow, M., & Lee, S. (2006). Enhanced biodegradation of diesel oil by a newly identified Rhodococcus baikonurensis EN3 in the presence of mycolic acid. Journal of Applied Microbiology, 100(2), (325-333). doi:10.1111/j.1365-2672.2005.02756.x. ISSN 1364-5072

Authors

Keywords

Biotechnology; Applied Microbiology and Biotechnology

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