Research Output

Intrafamilial genotyping of helicobacter pylori from faecal DNA.

  Helicobacter pylori infection, often acquired in early childhood, is a global cause of undernutrition, gastritis, peptic ulcer disease
and gastric carcinoma. This study tested the feasibility of using H. pylori shed in the faeces as a source of DNA for non-invasive
epidemiological studies. H. pylori DNA was chemically recovered and isolated using a specific biotinylated oligonucleotide probe
with magnetic capture from 28 H. pylori positive faecal samples obtained from children attending hospital for the investigation
of suspected H. pylori infection, together with close family members. Random amplification of polymorphic DNA (RAPD) was
subsequently used to discriminate each isolate. 93% of stool samples selected were typeable. Parent, child and sibling samples were compared and similarities determined. Phylogenetic analysis showed that H. pylori DNA obtained from the faeces can be used to
genotype individual strains, offering a means of studying intrafamilial transfer of this microorganism.

  • Type:

    Article

  • Date:

    01 July 2011

  • Publication Status:

    Published

  • Publisher

    Hindawi Limited

  • DOI:

    10.1155/2011/491035

  • Cross Ref:

    491035

  • ISSN:

    1687-6121

  • Library of Congress:

    R1 Medicine (General)

  • Dewey Decimal Classification:

    616 Diseases

  • Funders:

    Edinburgh Napier Funded

Citation

McMillan, M., MacKay, W. G., Williams, C. L., Shepherd, A. J., Malcolm, C., & Weaver, L. T. (2011). Intrafamilial genotyping of helicobacter pylori from faecal DNA. Gastroenterology Research and Practice, 2011, 1-7. https://doi.org/10.1155/2011/491035

Authors

Keywords

Hepatology; Gastroenterology,

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