Research Output
Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter
  A genomic clone containing the 5'-flanking sequence of the chicken {GABAA} receptor α1-subunit-encoding gene (GabRα1) was isolated and characterized. An intron was found to interrupt the 5'-untranslated region. The transcription start point (tsp) was determined by primer extension, {RNase} protection and the amplification of chick brain first-strand cDNA. {DNA} sequence analysis revealed a number of putative transcriptional regulatory motifs, including a {TATA} box 30 nucleotides upstream from the tsp, and that this region is a CpG island. While there is conservation between the chicken and human GabRα1 sequences, the chicken GabRα1 promoter has a different structure to those reported for the {GABAA} receptor β3- and δ-subunit-encoding genes.

  • Type:

    Article

  • Date:

    28 February 1995

  • Publication Status:

    Published

  • DOI:

    10.1016/0378-1119(94)00776-o

  • Cross Ref:

    037811199400776O

  • ISSN:

    0378-1119

  • Library of Congress:

    WL Cerebrovascular diseases,

  • Dewey Decimal Classification:

    616.8 Nervous & mental disorders

Citation

Bateson, A. N., Ultsch, A., & Darlison, M. G. (1995). Isolation and sequence analysis of the chicken GABAa receptor α1-subunit gene promoter. Gene, 153(2), 243-247. https://doi.org/10.1016/0378-1119%2894%2900776-o

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