Storrie, S., Longbottom, D., Barlow, P. G. & Wheelhouse, N. (2016). MAPK activation is essential for Waddlia chondrophila induced CXCL8 expression in human epithelial cells. PLoS ONE. 11, e0152193. doi:10.1371/journal.pone.0152193. ISSN 1932-6203
Waddlia chondrophila (W. chondrophila) is an emerging agent of respiratory and reproductive
disease in humans and cattle. The organism is a member of the order Chla...
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A. & Farquharson, C. (2016). E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models: PROTEASOME INHIBITION INCREASES E11 STABILITY. Journal of Cellular Physiology. 231(6), 1392-1404. doi:10.1002/jcp.25282. ISSN 0021-9541
The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are stil...
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2015). Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology. 145(1), 67-79. doi:10.1007/s00418-015-1365-8. ISSN 0948-6143
SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repa...
Chief Scientists Office
We have identified the human cathelicidn LL-37 as having potent direct antiviral activity against human rhinovirus (HRV), together with the capacity to alter death pathways in HRV-infected cells, as a...
Crohn's in Childhood Research Association
An investigation of commonly used IBD drugs on autophagy pathway activity and potential therapeutic benefit for treatment of paediatric IBD
Medical Research Council
Risks of developing many adult illnesses are set down as we develop in the womb, hence the environment we experience during fetal life needs to be ‘just right’. Understanding how the fetal environmen...
Senior Lecturer, Teaching Fellow, Head of Life Sciences
Lecturer and Research Leader in Biomedical Sciences
Huesa, C., Staines, K., Millán, J. & MacRae, V. (2015). Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy. International Journal of Molecular Medicine. 36(1), 159-165. doi:10.3892/ijmm.2015.2212. ISSN 1107-3756
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder of spontaneous infantile arterial and periarticular calcification which is attributed t...
Fraser, J. A., Worrall, E. G., Lin, Y., Landre, V., Pettersson, S., Blackburn, E., …Hupp, T. R. (2015). Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis. Journal of Molecular Biology. 427, 1728-1747. doi:10.1016/j.jmb.2014.12.011. ISSN 0022-2836
Mouse double minute 2 (MDM2) has a phosphorylation site within a lid motif at Ser17 whose phosphomimetic
mutation to Asp17 stimulates MDM2-mediated polyubiquitination of p53. ...
Dobie, R., Ahmed, S. F., Staines, K. A., Pass, C., Jasim, S., MacRae, V. E. & Farquharson, C. (2015). Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH. Journal of Cellular Physiology. 230(11), 2796-2806. doi:10.1002/jcp.25006. ISSN 0021-9541
Growth hormone (GH) signaling is essential for postnatal linear bone growth, but the relative importance of GHs actions on the liver and/or growth plate cartilage remains uncl...
Girardi, G., Fraser, J. A., Lennen, R., Vontell, R., Jansen, M. & Hutchison, G. (2014). Imaging of activated complement using ultrasmall superparamagnetic iron oxide particles (USPIO) - conjugated vectors: an in vivo in utero non-invasive method to predict placental insufficiency and abnormal fetal brain development. Molecular Psychiatry. 20, 1017-1026. doi:10.1038/mp.2014.110. ISSN 1359-4184
In the current study, we have developed a magnetic resonance imaging-based method for non-invasive detection of
complement activation in placenta and foetal brain in vivo in u...
Barlow, P. G., Findlay, E. G., Currie, S. M. & Davidson, D. J. (2013). Antiviral potential of cathelicidins. Future Microbiology. 9, 55-73. doi:10.2217/fmb.13.135. ISSN 1746-0913
The global burden of morbidity and mortality arising from viral infections is high; however, the development of effective therapeutics has been slow. As our understanding of i...
Wheelhouse, N., Coyle, C., Barlow, P. G., Mitchell, S., Greub, G., Baszler, T., …Longbottom, D. (2013). Waddlia chondrophila Infects and Multiplies in Ovine Trophoblast Cells Stimulating an Inflammatory Immune Response. PLoS ONE. 9, e102386. doi:10.1371/journal.pone.0102386. ISSN 1932-6203
Waddlia chondrophila (W. chondrophila) is an emerging abortifacient organism which has been identified in the placentae of humans and cattle. The organism is a mem...
Mackenzie, N. C. W., Staines, K. A., Zhu, D., Genever, P. & MacRae, V. E. (2014). miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION. Cell Biochemistry and Function. 32(2), 209-216. doi:10.1002/cbf.3005. ISSN 0263-6484
Vascular calcification shares many similarities with skeletal mineralisation and involves the phenotypic trans-differentiation of vascular smooth muscle cells (VSMCs) to osteo...
Staines, K. A., Pollard, A. S., McGonnell, I. M., Farquharson, C. & Pitsillides, A. A. (2013). Cartilage to bone transitions in health and disease. Journal of Endocrinology. 219(1), R1-R12. doi:10.1530/joe-13-0276. ISSN 0022-0795
Aberrant redeployment of the 'transient' events responsible for bone development and postnatal longitudinal growth has been reported in some diseases in what is otherwise inhe...
Staines, K. A., Zhu, D., Farquharson, C. & MacRae, V. E. (2014). Identification of novel regulators of osteoblast matrix mineralization by time series transcriptional profiling. Journal of Bone and Mineral Metabolism. 32(3), 240-251. doi:10.1007/s00774-013-0493-2. ISSN 0914-8779
Bone mineralization is a carefully orchestrated process, regulated by a number of promoters and inhibitors that function to ensure effective hydroxyapatite formation. Here we ...
Prach, M., Stone, V. & Proudfoot, L. (2012). Zinc oxide nanoparticles and monocytes: Impact of size, charge and solubility on activation status. Toxicology and Applied Pharmacology. 266(1), 19-26. doi:10.1016/j.taap.2012.10.020. ISSN 0041-008X
Zinc oxide (ZnO) particle induced cytotoxicity was dependent on size, charge and solubility, factors which at sublethal concentrations may influence the activation of the huma...
Newton, P. T., Staines, K. A., Spevak, L., Boskey, A. L., Teixeira, C. C., Macrae, V. E., …Farquharson, C. (2012). Chondrogenic ATDC5 cells: An optimised model for rapid and physiological matrix mineralisation. International Journal of Molecular Medicine. 5, 1187-93. doi:10.3892/ijmm.2012.1114. ISSN 1107-3756
The development of chondrogenic cell lines has led to major advances in the understanding of how chondrocyte differentiation is regulated, and has uncovered many signalling pa...
Staines, K. A., Mackenzie, N. C. W., Clarkin, C. E., Zelenchuk, L., Rowe, P. S., MacRae, V. E. & Farquharson, C. (2012). MEPE is a novel regulator of growth plate cartilage mineralization. BONE. 51(3), 418-430. doi:10.1016/j.bone.2012.06.022. ISSN 8756-3282
Matrix extracellular phosphoglycoprotein (MEPE) belongs to the SIBLING protein family which play key roles in biomineralization. Although the growth plates of MEPE-overexpress...
Society for Reproduction and Fertility
Does the gut microbiome play a role in polycystic ovary syndrome?
The University of Edinburgh
Neuromodulation can improve ovarian dysfunction in polycystic ovary syndrome (PCOS). This is achieved by four mechanisms: i) if neural signals are involved in LH action directly their modulation may ...
Undergraduate vacation scholarship application to study the legacy of prenatal programming of polycystic ovary syndrome on postnatal throid function
Polycystic ovary syndrome (PCOS) is common: more than one in 1 in 15 young women has this condition. There are many associated health problems including abnormal periods, miscarriages, infertility, ex...