Research Output
The site-specific Integration system of the temperate Streptococcus thermophiles Bacteriophage φSfi21
  The temperate bacteriophage fSfi21 integrates its DNA into the chromosome of Streptococcus thermophilus strains via
site-specific recombination. Nucleotide sequencing of the attachment sites identified a 40-bp identity region which surprisingly
overlaps both the 18-terminal bp of the phage integrase gene and the 11-terminal bp of a host tRNAArg gene. A 2.4-kb phage
DNA segment, covering attP, the phage integrase, and a likely immunity gene contained all the genetic information for faithful
integration of a nonreplicative plasmid into the attB site. A deletion within the int gene led to the loss of integration proficiency.
A number of spontaneous deletions were observed in plasmids containing the 2.4-kb phage DNA segment. The deletion
sites were localized to the tRNA side of the identity region and to phage or vector DNA with 3- to 6-bp-long repeats from
the border region. A similar type of deletion was previously observed in a spontaneous phage mutant.

  • Type:

    Article

  • Date:

    13 October 1997

  • Publication Status:

    Published

  • Publisher

    Elsevier

  • DOI:

    10.1006/viro.1997.8769

  • Cross Ref:

    S0042682297987699

  • ISSN:

    0042-6822

  • Library of Congress:

    QH301 Biology

  • Dewey Decimal Classification:

    579 Microorganisms, fungi & algae

Citation

Bruttin, A., Foley, S., & Brüssow, H. (1997). The site-specific Integration system of the temperate Streptococcus thermophiles Bacteriophage φSfi21. Virology, 237(1), 148-158. https://doi.org/10.1006/viro.1997.8769

Authors

Keywords

Temperate bacteriophage φSfi21; DNA; Streptococcus thermophilusstrains; recombination;

Monthly Views:

Available Documents