FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression
Journal Article
Ikpegbu, E., Basta, L., Clements, D. N., Fleming, R., Vincent, T. L., Buttle, D. J., …Farquharson, C. (2018)
FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression. Journal of Cellular Physiology, 233(7), 5334-5347. https://doi.org/10.1002/jcp.26345
E11/podoplanin is critical in the early stages of osteoblast-to-osteocyte transitions (osteocytogenesis) however the upstream events which regulate E11 expression are unknown....
Sost deficiency does not alter bone’s lacunar or vascular porosity in mice
Journal Article
Mosey, H., Núñez, J. A., Goring, A., Clarkin, C. E., Staines, K. A., Lee, P. D., …Javaheri, B. (2017)
Sost deficiency does not alter bone’s lacunar or vascular porosity in mice. Frontiers in Materials, 4, https://doi.org/10.3389/fmats.2017.00027
SCLEROSTIN (Sost) is expressed predominantly in osteocytes acting as a negative regulator of bone formation. In humans, mutations in the SOST gene lead to skeletal overgrowth ...
A distinctive patchy osteomalacia characterises Phospho1
-deficient mice
Journal Article
-deficient mice. Journal of Anatomy, 231(2), 298-308. https://doi.org/10.1111/joa.12628)
-deficient mice. Journal of Anatomy, 231(2), 298-308. https://doi.org/10.1111/joa.12628
The phosphatase PHOSPHO1 is involved in the initiation of biomineralisation. Bones in Phospho1 KO mice show histological osteomalacia with frequent bowing of long bones and sp...
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity
Journal Article
Poulet, B., Liu, K., Plumb, D., Vo, P., Shah, M., Staines, K., …Bou-Gharios, G. (2016)
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity. PLOS ONE, 11(12), https://doi.org/10.1371/journal.pone.0167971
Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme f...
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures
Journal Article
Houston, D. A., Myers, K., MacRae, V. E., Staines, K. A., & Farquharson, C. (2016)
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures. Calcified Tissue International, 99(5), 510-524. https://doi.org/10.1007/s00223-016-0176-9
Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, are catabolic to bone. The increase in the RANKL/OPG ratio in response to c...
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model
Journal Article
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016)
Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis and Rheumatology, 68(4), 880-891. https://doi.org/10.1002/art.39508
OBJECTIVE:
To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they a...
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
Journal Article
Houston, D. A., Staines, K. A., MacRae, V. E., & Farquharson, C. (2016)
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. Journal of Visualized Experiments, 118(118), https://doi.org/10.3791/54978
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth...
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models
Journal Article
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A., & Farquharson, C. (2016)
E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models. Journal of Cellular Physiology, 231(6), 1392-1404. https://doi.org/10.1002/jcp.25282
The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are stil...
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing
Journal Article
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2016)
Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology, 145(1), 67-79. https://doi.org/10.1007/s00418-015-1365-8
SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repa...
Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH
Journal Article
Dobie, R., Ahmed, S. F., Staines, K. A., Pass, C., Jasim, S., MacRae, V. E., & Farquharson, C. (2015)
Increased linear bone growth by GH in the absence of SOCS2 is independent of IGF-1: SOCS2 REGULATION OF GH INDUCED GROWTH. Journal of Cellular Physiology, 230(11), 2796-2806. https://doi.org/10.1002/jcp.25006
Growth hormone (GH) signaling is essential for postnatal linear bone growth, but the relative importance of GHs actions on the liver and/or growth plate cartilage remains uncl...