Houston, D. A., Staines, K. A., MacRae, V. E. & Farquharson, C. (2016). Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. Journal of Visualized Experiments. doi:10.3791/54978
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth...
Wheelhouse, N., Flockhart, A., Aitchison, K., Livingstone, M., Finlayson, J., Flachon, V., …Longbottom, D. (2016). Experimental challenge of pregnant cattle with the putative abortifacient Waddlia chondrophila. Scientific Reports. 6, 37150. doi:10.1038/srep37150. ISSN 2045-2322
Waddlia chondrophila is a Gram-negative intracellular bacterial organism that is related to classical chlamydial species and has been implicated as a cause of abortion in catt...
Dancer, S. J. (2016). Antibiotic prescribing: the consequences of getting it wrong. The Journal. 10(2), 62-71
Antibiotics were one of the most important discoveries last century. As with all drugs, however, they are associated with adverse effects. This article reviews how inappropria...
Chief Scientists Office
We have identified the human cathelicidn LL-37 as having potent direct antiviral activity against human rhinovirus (HRV), together with the capacity to alter death pathways in HRV-infected cells, as a...
Crohn's in Childhood Research Association
An investigation of commonly used IBD drugs on autophagy pathway activity and potential therapeutic benefit for treatment of paediatric IBD
Dancer, S. J. (2016). Dos and don’ts for hospital cleaning. Current Opinion in Infectious Diseases. 29(4), 415-423. doi:10.1097/qco.0000000000000289. ISSN 0951-7375
Purpose of review: More evidence is emerging on the role of cleaning and decontamination for reducing hospital-acquired infection. Timely and adequate removal of environmental...
Houston, D. A., Myers, K., MacRae, V. E., Staines, K. A. & Farquharson, C. (2016). The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures. Calcified Tissue International. 99(5), 510-524. doi:10.1007/s00223-016-0176-9. ISSN 0171-967X
Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, are catabolic to bone. The increase in the RANKL/OPG ratio in response to c...
Barlow, P. G., Coyle, C., Wheelhouse, N., Jacques, M., Longbottom, D., Svoboda, P., …Colin Duncan, W. (2016). Ovine trophoblasts express cathelicidin host defence peptide in response to infection. Journal of Reproductive Immunology. 117, 10-16. doi:10.1016/j.jri.2016.06.006. ISSN 0165-0378
Cationic host defence peptides (CHDP; also known as antimicrobial peptides) are key components of the immune response in the female reproductive tract. The role of the placent...
Sousa, F. H., Casanova, V., Stevens, C. & Barlow, P. G. (2016). Antiviral host defence peptides. In Epand, R. M. (Ed.). Host Defense Peptides and Their Potential as Therapeutic Agents, 57-94. Springer. doi:10.1007/978-3-319-32949-9_3. ISBN 978-3-319-32947-5
The on going global mortality and morbidity associated with viral pathogens highlights the need for the continued development of effective, novel antiviral molecules. The anti...
Simpson, R. J., Graham, S. M., Connaboy, C., Clement, R., Pollonini, L. & Florida-James, G. (2016). Blood lactate thresholds and walking/running economy are determinants of backpack-running performance in trained soldiers. Applied Ergonomics. 58, 566-572. doi:10.1016/j.apergo.2016.04.010. ISSN 0003-6870
We developed a standardized laboratory treadmill protocol for assessing physiological responses to a simulated backpack load-carriage task in trained soldiers, and assessed th...
Staines, K. A., Madi, K., Mirczuk, S. M., Parker, S., Burleigh, A., Poulet, B., …Pitsillides, A. A. (2016). Endochondral Growth Defect and Deployment of Transient Chondrocyte Behaviors Underlie Osteoarthritis Onset in a Natural Murine Model. Arthritis and Rheumatology. 68(4), 880-891. doi:10.1002/art.39508. ISSN 2326-5191
To explore whether aberrant transient chondrocyte behaviors occur in the joints of STR/Ort mice (which spontaneously develop osteoarthritis [OA]) and whether they a...
Storrie, S., Longbottom, D., Barlow, P. G. & Wheelhouse, N. (2016). MAPK activation is essential for Waddlia chondrophila induced CXCL8 expression in human epithelial cells. PLoS ONE. 11, e0152193. doi:10.1371/journal.pone.0152193. ISSN 1932-6203
Waddlia chondrophila (W. chondrophila) is an emerging agent of respiratory and reproductive
disease in humans and cattle. The organism is a member of the order Chla...
Staines, K. A., Prideaux, M., Allen, S., Buttle, D. J., Pitsillides, A. A. & Farquharson, C. (2016). E11/Podoplanin Protein Stabilization Through Inhibition of the Proteasome Promotes Osteocyte Differentiation in Murine in Vitro Models: PROTEASOME INHIBITION INCREASES E11 STABILITY. Journal of Cellular Physiology. 231(6), 1392-1404. doi:10.1002/jcp.25282. ISSN 0021-9541
The transmembrane glycoprotein E11 is considered critical in early osteoblast–osteocyte transitions (osteocytogenesis), however its function and regulatory mechanisms are stil...
Zaman, G., Farquharson, C., Staines, K. A., Newton, P. T., Dudhia, J., Chenu, C., …Dhoot, G. K. (2015). Expression of Sulf1 and Sulf2 in cartilage, bone and endochondral fracture healing. Histochemistry and Cell Biology. 145(1), 67-79. doi:10.1007/s00418-015-1365-8. ISSN 0948-6143
SULF1/SULF2 enzymes regulate cell signalling that impacts the growth and differentiation of many tissues. To determine their possible role in cartilage and bone growth or repa...
Huesa, C., Staines, K., Millán, J. & MacRae, V. (2015). Effects of etidronate on the Enpp1-/- mouse model of generalized arterial calcification of infancy. International Journal of Molecular Medicine. 36(1), 159-165. doi:10.3892/ijmm.2015.2212. ISSN 1107-3756
Generalized arterial calcification of infancy (GACI) is an autosomal recessive disorder of spontaneous infantile arterial and periarticular calcification which is attributed t...
Fraser, J. A., Worrall, E. G., Lin, Y., Landre, V., Pettersson, S., Blackburn, E., …Hupp, T. R. (2015). Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis. Journal of Molecular Biology. 427, 1728-1747. doi:10.1016/j.jmb.2014.12.011. ISSN 0022-2836
Mouse double minute 2 (MDM2) has a phosphorylation site within a lid motif at Ser17 whose phosphomimetic
mutation to Asp17 stimulates MDM2-mediated polyubiquitination of p53. ...