Research Output
A critical analysis of the combined usage of protein localization prediction methods: Increasing the number of independent data sets can reduce the accuracy of predicted mitochondrial localization
  In the absence of a comprehensive experimentally derived mitochondrial proteome, several bioinformatic approaches have been developed to aid the identification of novel mitochondrial disease genes within mapped nuclear genetic loci. Often, many classifiers are combined to increase the sensitivity and specificity of the predictions.

Here we show that the greatest sensitivity and specificity are obtained by using a combination of seven carefully selected classifiers. We also show that increasing the number of independent prediction methods can paradoxically decrease the accuracy of predicting mitochondrial localization. This approach will help to accelerate the identification of new mitochondrial disease genes by providing a principled way for the selection for combination of appropriate prediction methods of mitochondrial localization of proteins.

  • Type:

    Article

  • Date:

    31 December 2010

  • Publication Status:

    Published

  • Publisher

    Elsevier

  • DOI:

    10.1016/j.mito.2010.12.016

  • ISSN:

    1567-7249

  • Funders:

    Historic Funder (pre-Worktribe)

Citation

Lythgow, K. T., Hudson, G., Andras, P., & Chinnery, P. F. (2011). A critical analysis of the combined usage of protein localization prediction methods: Increasing the number of independent data sets can reduce the accuracy of predicted mitochondrial localization. Mitochondrion, 11(3), 444-449. https://doi.org/10.1016/j.mito.2010.12.016

Authors

Keywords

Mitochondria, Proteome, Bioinformatics, Oxidative phosphorylation, Mitochondrial disease

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