Research Output
Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes
  We have amplified partial complementary {DNAs} for the chicken γ-aminobutyric acid type C (GABAC) receptor ρ1 and ρ2 subunits using the polymerase chain reaction. These nucleotide sequences have been utilized to design specific oligonucleotide probes for the in situ hybridization localization of the corresponding messenger {RNAs} (mRNAs) in the 1-day-old chick retina. Although both transcripts are found almost exclusively in the inner nuclear layer, their distributions differ markedly. From the locations of the hybridization signals, we deduce that the ρ1-subunit mRNA is present mainly in bipolar cells and that the ρ2-subunit mRNA is present in both amacrine and horizontal cells. These results suggest that the ρ1 and ρ2 subunits frequently occur in different receptor complexes and, therefore, that subtypes of the {GABAC} receptor exist.

  • Type:

    Article

  • Date:

    21 April 1995

  • Publication Status:

    Published

  • DOI:

    10.1016/0304-3940(95)11479-g

  • Cross Ref:

    030439409511479G

  • ISSN:

    0304-3940

  • Library of Congress:

    QP Physiology

  • Dewey Decimal Classification:

    572 Biochemistry

Citation

Albrecht, B. E., & Darlison, M. G. (1995). Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes. Neuroscience Letters, 189(3), 155-158. https://doi.org/10.1016/0304-3940%2895%2911479-g

Keywords

General Neuroscience

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