Research Output
Molecular identification and characterization of mannitol-negative methicillin-resistant Staphylococcus aureus
  We report on the isolation, molecular identification, and characterization of 5 mannitol-negative methicillin-resistant Staphylococcus aureus (MRSA) from clinical samples in KwaZulu-Natal (KZN) province, South Africa. Identification based on phenotypic testing and polymerase chain reaction detection of the S. aureus species-specific nuc gene and the coagulase gene indicated that the mannitol-negative isolates were S. aureus. Furthermore, they were mecA positive, and SCCmec typing demonstrated that all the isolates harbored type IV SCCmec. API STAPH (Biomerieux, Marcy-l'Etoile, France) misidentified 2 mannitol-negative MRSA that belonged to the major clone in KZN province, as Staphylococcus lugdunensis. Although the prevalence and mechanism of mannitol-negative MRSA is unknown, laboratories are encouraged to investigate S. aureus with atypical characteristics.

  • Type:

    Article

  • Date:

    18 July 2006

  • Publication Status:

    Published

  • Publisher

    Elsevier BV

  • DOI:

    10.1016/j.diagmicrobio.2006.05.004

  • Cross Ref:

    S0732889306001970

  • ISSN:

    0732-8893

  • Funders:

    Historic Funder (pre-Worktribe)

Citation

Shittu, A., Lin, J., & Morrison, D. (2007). Molecular identification and characterization of mannitol-negative methicillin-resistant Staphylococcus aureus. Diagnostic Microbiology and Infectious Disease, 57(1), 93-95. https://doi.org/10.1016/j.diagmicrobio.2006.05.004

Authors

Keywords

Characterization; Mannitol-negative; MSRA

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