Research Output
The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase
  Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used “sports”/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki’ = 24.9mM) and L-valine, the least potent (Ki’ = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.

  • Type:

    Article

  • Date:

    06 November 2018

  • Publication Status:

    Published

  • Publisher

    Canadian Science Publishing

  • DOI:

    10.1139/apnm-2018-0449

  • Cross Ref:

    10.1139/apnm-2018-0449

  • ISSN:

    1715-5312

  • Library of Congress:

    QP Physiology

  • Dewey Decimal Classification:

    571 Physiology & related subjects

  • Funders:

    ENU Development Trust

Citation

Boyd, G. W., Drew, M., Ward, S., Baird, M., Graham, S. M., & Connaboy, C. (2018). The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase. Applied Physiology, Nutrition, and Metabolism, https://doi.org/10.1139/apnm-2018-0449

Authors

Keywords

Nutrition and Dietetics; Physiology (medical); Physiology; Endocrinology, Diabetes and Metabolism; General Medicine

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