Research Output
The Ex Vivo Organ Culture of Bone
  The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of processes and activities that are fundamental to bone and cartilage biology. Through maintaining the bone and/or cartilage cells in their native environment, this model system provides the investigator with a powerful experimental protocol to address specific facets of skeletal growth and development. In this chapter, we outline the basic protocols and possible readouts of organ culture models to replicate; (a) linear bone growth (murine metatarsal culture model), (b) bone and cartilage metabolism (murine femoral head culture model), (c) bone response to mechanical stimulation (bovine trabecular core culture model), and (d) bone resorption and formation (murine calvaria culture model).

  • Date:

    08 February 2019

  • Publication Status:

    Published

  • Publisher

    Springer New York

  • DOI:

    10.1007/978-1-4939-8997-3_10

  • ISSN:

    1064-3745

  • Library of Congress:

    QH301 Biology

  • Dewey Decimal Classification:

    572 Biochemistry

  • Funders:

    Biotechnology and Biological Sciences Research Council

Citation

Staines, K. A., Brown, G., & Farquharson, C. (2019). The Ex Vivo Organ Culture of Bone. In Bone Research Protocols (199-215). New york, USA: Humana Press. https://doi.org/10.1007/978-1-4939-8997-3_10

Authors

Keywords

Metatarsal, calvaria, femoral head, Trabecular core, organ culture,

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