Research Output
The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit
  We have produced a stable insect cell line derived from Spodoptera frugiperda (Sf9) cells expressing a cDNA encoding a β-subunit of the Lymnaea stagnalis GABAA receptor. The cDNA was randomly integrated into the insect cell genome under the control of a baculovirus immediate early gene (IE-1) promoter. Stable cell lines were established by transformation of Sf9 cells with the expression vector pIEK1.LGβ1 together with a plasmid encoding a selectable marker which confers neomycin (G418) resistance. Following growth in the presence of G418, neomycin resistant clones were selected, amplified and analysed for the presence of functional GABA-gated chloride channels. Electrophysiological analysis of one cell line showed the presence of a picrotoxin-sensitive chloride channel not present in control Sf9 cells. These channels were also sensitive to GABA, albeit at relatively high (mM) concentrations.

  • Type:

    Article

  • Date:

    31 January 1995

  • Publication Status:

    Published

  • Publisher

    Informa UK Limited

  • DOI:

    10.3109/10799899509045205

  • Cross Ref:

    10.3109/10799899509045205

  • ISSN:

    1079-9893

  • Library of Congress:

    QH301 Biology

  • Dewey Decimal Classification:

    572 Biochemistry

Citation

Smith, L. A., Amar, M., Harvey, R. J., Darlison, M. G., Earley, F. G. P., Beadle, D. J., …Bermudez, I. (1995). The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit. Journal of Receptors and Signal Transduction, 15(1-4), 33-41. https://doi.org/10.3109/10799899509045205

Keywords

Spodoptera frugiperda (Sf9), Stable cell lines, plasmid encoding, Electrophysiological analysis,

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