Research Output
Growth hormone and amino acid supply interact synergistically to control insulin-like growth factor-I production and gene expression in cultured ovine hepatocytes.
  Many of the anabolic effects of growth hormone (GH) are indirect, occurring through GH-stimulated production of insulin-like growth factor-I (IGF-I) by the liver. As well as being regulated by GH, plasma IGF-I concentrations have been demonstrated to depend upon the level of dietary protein intake, with low protein diets being associated with reduced circulatory IGF-I levels. This inhibitory effect cannot be reversed by GH injection, suggesting that liver sensitivity to GH becomes impaired. To investigate the mechanisms through which protein supply affects GH sensitivity, primary cultures of ovine hepatocytes were grown in defined media, containing various proportions (0.2, 1.0 and 5.0) of jugular amino acid concentrations in fed sheep. Production of IGF-I by these cells was measured after 24 and 48 h in culture by radioimmunoassay. In the first 24-h period basal IGF-I production was the same in all defined media, and GH caused an approximately 2-fold increase in IGF-I release in cells grown in 1.0xor 5.0xamino acid media (P

  • Type:

    Article

  • Date:

    01 November 1999

  • Publication Status:

    Published

  • DOI:

    10.1677/joe.0.1630353

  • ISSN:

    0022-0795

  • Library of Congress:

    QL Zoology

  • Dewey Decimal Classification:

    590 Animals (Zoology)

Citation

Wheelhouse, N. M., Stubbs, A. K., Lomax, M. A., & MacRae, J. C. (1999). Growth hormone and amino acid supply interact synergistically to control insulin-like growth factor-I production and gene expression in cultured ovine hepatocytes. Journal of Endocrinology, 163(2), 353-361. https://doi.org/10.1677/joe.0.1630353

Authors

Keywords

Ovine, amino acid, growth hormone,

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