Research Output

Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment

  Tubal ectopic pregnancies are a leading cause of global maternal morbidity and mortality. Previous infection with Chlamydia trachomatis is a major risk factor for tubal embryo implantation but the biological mechanism behind this association is unclear. Successful intra-uterine embryo implantation is associated with increased expression of endometrial “receptivity” integrins (cell adhesion molecules).We examined integrin expression in Fallopian
tubes of women with previous C. trachomatis infection, in mice experimentally infected with C. trachomatis, in immortalised human oviductal epithelial cells (OE-E6/E7) and in an in vitro model of human embryo attachment (trophoblast spheroid-OE-E6/7 cell co-culture). Previous exposure with C. trachomatis increased Fallopian tube/oviduct integrin-subunit beta-1 (ITGB1) in women and mice compared to controls. C. trachomatis increased OEE6/E7 cell ITGB1 expression and promoted trophoblast attachment to OE-E6/E7 cellswhichwas negated by anti-ITGB1-antibody.We demonstrate that infection with C. trachomatis increases tubal ITGB1 expression, predisposing to tubal embryo attachment and ectopic pregnancy.

  • Type:

    Article

  • Date:

    23 February 2018

  • Publication Status:

    Published

  • Publisher

    Elsevier BV

  • DOI:

    10.1016/j.ebiom.2018.02.020

  • Cross Ref:

    S2352396418300744

  • Library of Congress:

    RG Gynecology and obstetrics

  • Dewey Decimal Classification:

    618 Gynecology, obstetrics, pediatrics & geriatrics

  • Funders:

    Edinburgh Napier Funded

Citation

Ahmad, S. F., Brown, J. K., Campbell, L. L., Koscielniak, M., Oliver, C., Wheelhouse, N., …Horne, A. W. (2018). Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment. EBioMedicine, doi:10.1016/j.ebiom.2018.02.020

Authors

Keywords

Ectopic pregnancy, Chlamydia trachomatis, Integrins, embryo implantation, fallopian tube

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