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Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants

  The formation of viscous foams on aeration basins and secondary clarifiers of activated sludge plants is a common and widespread problem. Foam formation is often attributed to the presence of mycolic acid-containing actinomycetes (mycolata). In order to examine the relationship between the number of mycolata and foam, we developed a group-specific probe targeting the 16S rRNA of the mycolata, a protocol to permeabilize mycolata, and a statistically robust quantification method. Statistical analyses showed that a lipase-based permeabilization method was quantitatively superior to previously described methods (P

  • Type:

    Article

  • Date:

    01 March 2000

  • Publication Status:

    Published

  • Publisher

    American Society for Microbiology

  • DOI:

    10.1128/aem.66.3.1158-1166.2000

  • ISSN:

    0099-2240

  • Library of Congress:

    QR Microbiology

  • Dewey Decimal Classification:

    579 Microorganisms, fungi & algae

Citation

Davenport, R. J., Curtis, T. P., Goodfellow, M., Stainsby, F. M., & Bingley, M. (2000). Quantitative Use of Fluorescent In Situ Hybridization To Examine Relationships between Mycolic Acid-Containing Actinomycetes and Foaming in Activated Sludge Plants. Applied and Environmental Microbiology, 66(3), 1158-1166. doi:10.1128/aem.66.3.1158-1166.2000

Authors

Keywords

Biotechnology; Food Science; Ecology; Applied Microbiology and Biotechnology

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