Research Output

The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy

  The cytoskeletal protein vimentin plays a key role in positioning of organelles within the cytosol and has been linked to the regulation of numerous cellular processes including autophagy, however, how vimentin regulates autophagy remains relatively unexplored. Here we report that inhibition of vimentin using the steroidal lactone Withaferin A (WFA) causes vimentin to aggregate, and this is associated with the relocalisation of organelles including autophagosomes and lysosomes from the cytosol to a juxtanuclear location. Vimentin inhibition causes autophagosomes to accumulate, and we demonstrate this results from modulation of mechanistic target of rapamycin (mTORC1) activity, and disruption of autophagosome-lysosome fusion. We suggest that vimentin plays a physiological role in autophagosome and lysosome positioning, thus identifying vimentin as a key factor in the regulation of mTORC1 and autophagy.

  • Type:

    Article

  • Date:

    30 January 2019

  • Publication Status:

    Published

  • DOI:

    10.1371/journal.pone.0209665

  • ISSN:

    1932-6203

  • Library of Congress:

    QR180 Immunology

  • Dewey Decimal Classification:

    616 Diseases

  • Funders:

    Edinburgh Napier Funded

Citation

Biskou, O., Casanova, V., Hooper, K., Kemp, S., Wright, G. P., Satsangi, J., …Stevens, C. (2019). The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy. PLOS ONE, 14(1), https://doi.org/10.1371/journal.pone.0209665

Authors

Keywords

Vimentin; autophagy; lysosomes; autophagosomes; mTORC1;

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