The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit
Journal Article
Smith, L. A., Amar, M., Harvey, R. J., Darlison, M. G., Earley, F. G. P., Beadle, D. J., …Bermudez, I. (1995)
The Production of a Stably Transformed Insect Cell Line Expressing An Invertebrate GABA A Receptor β-Subunit. Journal of Receptors and Signal Transduction, 15(1-4), 33-41. https://doi.org/10.3109/10799899509045205
We have produced a stable insect cell line derived from Spodoptera frugiperda (Sf9) cells expressing a cDNA encoding a β-subunit of the Lymnaea stagnalis GABAA receptor. The c...
Molluscan ligand-gated ion-channel receptors
Book Chapter
Darlison, M. G., Hutton, M. L., & Harvey, R. J. (1993)
Molluscan ligand-gated ion-channel receptors. In Comparative Molecular Neurobiology, 48-64. Springer Verlag. https://doi.org/10.1007/978-3-0348-7265-2_3
In this chapter we introduce the reader to the structures of the different types of ligand-gated ion-channel receptor, and the numerous receptor subtypes that have recently be...
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique
Journal Article
Lasham, A., & Darlison, M. G. (1993)
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009
We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not requir...
Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors
Journal Article
Atkinson, A. E., Bermudez, I., Darlison, M. G., Barnard, E. A., Earley, F. G. P., Possee, R. D., …King, L. A. (1992)
Assembly of functional GABAA receptors in insect cells using baculovirus expression vectors. NeuroReport, 3(7), 597-600. https://doi.org/10.1097/00001756-199207000-00013
We have constructed recombinant baculoviruses containing cDNAs encoding either the [alpha]1- or the [beta]1-subunit of the bovine GABAA receptor. In Spodoptera frugiperda (IPL...
Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain
Book Chapter
Squire, M. D., Moss, S. J., Lai, F. A., Darlison, M. G., Cockcroft, V. B., Barnard, E. A., …Ross, S. J. (1986)
Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain. In A. Maelicke (Ed.), Nicotinic Acetylcholine Receptor, 389-415. Springer Verlag. https://doi.org/10.1007/978-3-642-71649-2_30
While it has been completely established that the AChR** as isolated from the electroplaques of electric fish is composed of four different polypeptides, such that its subunit...
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase ofEscherichia coli
Journal Article
Darlison, M. G., & Guest, J. R. (1984)
Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase ofEscherichia coli. Biochemical Journal, 223(2), 507-517. https://doi.org/10.1042/bj2230507
The nucleotide sequence of a 961 base-pair segment of DNA containing the sdhB gene, which encodes the iron-sulphur protein subunit of the E. coli succinate dehydrogenase, has ...
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli
Journal Article
Wood, D., Darlison, M. G., Wilde, R. J., & Guest, J. R. (1984)
Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli. The biochemical journal, 222(2), 519-534. https://doi.org/10.1042/bj2220519
The nucleotide sequence of a 3614 base-pair segment of DNA containing the sdhA gene, encoding the flavoprotein subunit of succinate dehydrogenase of Escherichia coli, and two ...
Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase
Journal Article
Spencer, M. E., Darlison, M. G., Stephens, P. E., Duckenfield, I. K., & Guest, J. R. (1984)
Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase. European journal of biochemistry / FEBS, 141(2), 361-374. https://doi.org/10.1111/j.1432-1033.1984.tb08200.x
The nucleotide sequence of the sucB gene, which encodes the dihydrolipoamide succinyltransferase component (E2o) of the 2‐oxoglutarate dehydrogenase complex of Escherichia col...
Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12
Journal Article
Darlison, M. G., Spencer, M. E., & Guest, J. R. (1984)
Nucleotide sequence of the sucA gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12. European journal of biochemistry / FEBS, 141(2), 351-359. https://doi.org/10.1111/j.1432-1033.1984.tb08199.x
The nucleotide sequence of a 3180‐base‐pair segment of DNA, containing the sucA gene encoding the 2‐oxoglutarate dehydrogenase component (E1o) of the 2‐oxoglutarate dehydrogen...
Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12
Journal Article
Stephens, P. E., Lewis, H. M., Darlison, M. G., & Guest, J. R. (1983)
Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12. European journal of biochemistry / FEBS, 135(3), 519-527. https://doi.org/10.1111/j.1432-1033.1983.tb07683.x
The nucleotide sequence of a 1980‐base‐pair segment of DNA, containing the lpd gene encoding the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase complex o...