The Ex Vivo Organ Culture of Bone
Book Chapter
Staines, K. A., Brown, G., & Farquharson, C. (2019)
The Ex Vivo Organ Culture of Bone. In Bone Research Protocols (199-215). New york, USA: Humana Press. https://doi.org/10.1007/978-1-4939-8997-3_10
The ex vivo organ culture of bone provides many of the advantages of both the whole organism and isolated cell strategies and can deliver valuable insight into the network of ...
MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells
Journal Article
Storrie, S., Longbottom, D., Barlow, P. G., & Wheelhouse, N. (2016)
MAPK Activation Is Essential for Waddlia chondrophila Induced CXCL8 Expression in Human Epithelial Cells. PLOS ONE, 11(3), https://doi.org/10.1371/journal.pone.0152193
Background
Waddlia chondrophila (W. chondrophila) is an emerging agent of respiratory and reproductive disease in humans and cattle. The organism is a member of the order Chla...
Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis
Journal Article
Fraser, J. A., Worrall, E. G., Lin, Y., Landre, V., Pettersson, S., Blackburn, E., …Hupp, T. R. (2015)
Phosphomimetic Mutation of the N-Terminal Lid of MDM2 Enhances the Polyubiquitination of p53 through Stimulation of E2-Ubiquitin Thioester Hydrolysis. Journal of Molecular Biology, 427(8), 1728-1747. https://doi.org/10.1016/j.jmb.2014.12.011
Mouse double minute 2 (MDM2) has a phosphorylation site within a lid motif at Ser17 whose phosphomimetic
mutation to Asp17 stimulates MDM2-mediated polyubiquitination of p53. ...
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2
Journal Article
Stevens, C., Pettersson, S., Wawrzynow, B., Wallace, M., Ball, K., Zylicz, A., & Hupp, T. R. (2008)
ATP stimulates MDM2-mediated inhibition of the DNA-binding function of E2F1: ATP stimulated inhibition of E2F1 by MDM2. FEBS Journal, 275(19), 4875-4886. https://doi.org/10.1111/j.1742-4658.2008.06627.x
Murine double minute 2 (MDM2) protein exhibits many diverse biochemical functions on the tumour suppressor protein p53, including transcriptional suppression and E3 ubiquitin ...
The MDM2 Ubiquitination Signal in the DNA-Binding Domain of p53 Forms a Docking Site for Calcium Calmodulin Kinase Superfamily Members
Journal Article
Craig, A. L., Chrystal, J. A., Fraser, J. A., Sphyris, N., Lin, Y., Harrison, B. J., …Hupp, T. R. (2007)
The MDM2 Ubiquitination Signal in the DNA-Binding Domain of p53 Forms a Docking Site for Calcium Calmodulin Kinase Superfamily Members. Molecular and Cellular Biology, 27(9), 3542-3555. https://doi.org/10.1128/mcb.01595-06
Genetic and biochemical studies have shown that Ser20 phosphorylation in the transactivation domain of p53 mediates p300-catalyzed DNA-dependent p53 acetylation and B-cell tum...
Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells
Journal Article
Hillier, S. G., Rae, M. T., Niven, D., Critchley, H. O. D., & Harlow, C. R. (2004)
Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells. Journal of Clinical Endocrinology and Metabolism, 89(9), 4538-4544. https://doi.org/10.1210/jc.2003-032225
The human ovarian surface epithelium (OSE) is subject to
serial injury and repair during ovulation, which is a natural inflammatory event. We asked whether there is a compensa...
The Modifier Subunit of Drosophila Glutamate-Cysteine Ligase Regulates Catalytic Activity by Covalent and Noncovalent Interactions and Influences Glutathione Homeostasis in Vivo
Journal Article
Fraser, J. A., Kansagra, P., Kotecki, C., Saunders, R. D. C., & McLellan, L. I. (2003)
The Modifier Subunit of Drosophila Glutamate-Cysteine Ligase Regulates Catalytic Activity by Covalent and Noncovalent Interactions and Influences Glutathione Homeostasis in Vivo. Journal of Biological Chemistry, 278(47), 46369-46377. https://doi.org/10.1074/jbc.m308035200
Glutamate-cysteine ligase (GCL) has a key influence on glutathione homeostasis. It has been proposed that mammalian GCL is regulated by the redox environment, and we show here...
Chk2 activates E2F-1 in response to DNA damage
Journal Article
Stevens, C., Smith, L., & La Thangue, N. B. (2003)
Chk2 activates E2F-1 in response to DNA damage. Nature Cell Biology, 5(5), 401-409. https://doi.org/10.1038/ncb974
The E2F-1 transcription factor is regulated during cell cycle progression and induced by cellular stress, such as DNA damage. We report that checkpoint kinase 2 (Chk2) regulat...
Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells
Journal Article
Sakka, E., Rae, M., Bramley, T., & Aitken, J. (1997)
Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells. Biology of Reproduction, 57(2), 428-435
We measured the production of reactive oxygen species (ROS) using luminol-horseradish peroxidase-induced chemiluminescence in mechanically dispersed cell suspensions from bovi...
Molluscan ligand-gated ion-channel receptors
Book Chapter
Darlison, M. G., Hutton, M. L., & Harvey, R. J. (1993)
Molluscan ligand-gated ion-channel receptors. In Comparative Molecular Neurobiology, 48-64. Springer Verlag. https://doi.org/10.1007/978-3-0348-7265-2_3
In this chapter we introduce the reader to the structures of the different types of ligand-gated ion-channel receptor, and the numerous receptor subtypes that have recently be...