38 results

Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection

Journal Article
Casanova, V., Sousa, F. H., Shakamuri, P., Svoboda, P., Buch, C., D'Acremont, M., …Barlow, P. G. (2020)
Citrullination Alters the Antiviral and Immunomodulatory Activities of the Human Cathelicidin LL-37 During Rhinovirus Infection. Frontiers in Immunology, 11, https://doi.org/10.3389/fimmu.2020.00085
Human rhinoviruses (HRV) are the most common cause of viral respiratory tract infections. While normally mild and self-limiting in healthy adults, HRV infections are associate...

Peptide Combinatorial Libraries Identify TSC2 as a Death-associated Protein Kinase (DAPK) Death Domain-binding Protein and Reveal a Stimulatory Role for DAPK in mTORC1 Signaling

Journal Article
Stevens, C., Lin, Y., Harrison, B., Burch, L., Ridgway, R. A., Sansom, O. J., & Hupp, T. R. (2009)
Peptide Combinatorial Libraries Identify TSC2 as a Death-associated Protein Kinase (DAPK) Death Domain-binding Protein and Reveal a Stimulatory Role for DAPK in mTORC1 Signaling. Journal of Biological Chemistry, 284, 334-344. https://doi.org/10.1074/jbc.M805165200
Death-associated protein kinase (DAPK) is a multidomain enzyme that plays a central role in autophagic and apoptotic signaling, although the protein-protein interactions regul...

Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis.

Journal Article
Lin, Y., Stevens, C., & Hupp, T. (2007)
Identification of a dominant negative functional domain on DAPK-1 that degrades DAPK-1 protein and stimulates TNFR-1-mediated apoptosis. Journal of Biological Chemistry, 282(23), 16792-16802. https://doi.org/10.1074/jbc.M611559200
DAPK-1 is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. To define mechanisms of DAPK-1 prot...

Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo

Journal Article
Mathur, S., Turnbull, A., Akaev, I., Stevens, C., Agrawal, N., Chopra, M., & Mincher, D. (2020)
Design of a New Peptide Substrate Probe of the Putative Biomarker Legumain with Potential Application in Prostate Cancer Diagnosis ex vivo. International Journal of Peptide Research and Therapeutics, 26, 1965-1980. https://doi.org/10.1007/s10989-019-09994-1
The lysosomal endoprotease legumain (asparaginyl endoprotease) has been proposed as a putative biomarker in prostate tumours, in which the enzyme is markedly overexpressed. Ov...

A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis

Journal Article
Stevens, C., Lin, Y., Sanchez, M., Amin, E., Copson, E., White, H., …Hupp, T. (2007)
A Germ Line Mutation in the Death Domain of DAPK-1 Inactivates ERK-induced Apoptosis. Journal of Biological Chemistry, 282(18), 13791-13803. https://doi.org/10.1074/jbc.m605649200
p53 is activated genetically by a set of kinases that are components of the calcium calmodulin kinase superfamily, including CHK2, AMP kinase, and DAPK-1. In dissecting the me...

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Journal Article
Klionsky, D. J., Kamal Abdel-Aziz, A., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., …et al., . (2021)
Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy, 17(1), 1-382. https://doi.org/10.1080/15548627.2020.1797280
In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have e...

Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease

Journal Article
Morgan, S., Hooper, K. M., Milne, E. M., Farquharson, C., Stevens, C., & Staines, K. A. (2019)
Azathioprine Has a Deleterious Effect on the Bone Health of Mice with DSS-Induced Inflammatory Bowel Disease. International Journal of Molecular Sciences, 20(23), https://doi.org/10.3390/ijms20236085
Patients with inflammatory bowel disease (IBD) often present poor bone health and are 40% more at risk of bone fracture. Studies have implicated autophagy in IBD pathology and...

The emerging role of E2F-1 in the DNA damage response and checkpoint control.

Journal Article
Stevens, C., & La Thangue, N. B. (2004)
The emerging role of E2F-1 in the DNA damage response and checkpoint control. DNA Repair, 3(8-9), 1071-1079. https://doi.org/10.1016/j.dnarep.2004.03.034
Genotoxic stress triggers a myriad of cellular responses including cell cycle arrest, stimulation of {DNA} repair and apoptosis. A central role for the E2F-1 transcription fac...

Death‐associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death.

Journal Article
Bovellan, M., Fritzsche, M., Stevens, C., & Charras, G. (2009)
Death‐associated protein kinase (DAPK) and signal transduction: blebbing in programmed cell death. FEBS Journal, 277(1), (58-65). ISSN 1742-464X
Death-associated protein kinase (DAPK) is a stress-regulated protein kinase that mediates a range of processes, including signal-induced cell death and autophagy. Although the...

A New Role for E2F-1 in Checkpoint Control

Journal Article
Stevens, C., & Thangue, N. B. L. (2003)
A New Role for E2F-1 in Checkpoint Control. Cell Cycle, 2(5), 434-436. https://doi.org/10.4161/cc.2.5.462
In response to DNA damage, E2F-1 is induced and phosphorylated. Phosphorylated E2F-1 can reside in discrete nuclear structures and induce apoptosis, suggesting a unique role f...
6 results

An investigation of commonly used IBD drugs on autophagy pathway activity and potential therapeutic benefit for the treatment of paediatric IBD

2014 - 2017
Recent genetic discoveries, principally identification of NOD2 and ATG16L1 as susceptibility loci, strongly implicate a dysregulated host response to enteric bacteria and the autophagy pathway in the ...
Funder: Crohn's in Childhood Research Association | Value: £68,577

Host Defence Peptides as novel modulators of autophagy in Inflammatory Bowel Disease

2019 - 2022
Increasing numbers of patients are diagnosed with inflammatory bowel disease (IBD) each year, both in the United Kingdom and worldwide. Most drugs in current use for the treatment of IBD target the im...
Funder: The Daphne Jackson Trust | Value: £83,167

Cathelicidins as Novel Therapeutic Antivirals for Dengue Infection

2019 - 2022
A significant challenge faced by modern society is the lack of therapeutics for a large number of infections, particularly those that are endemic or widely spread in developing countries. Dengue is on...
Funder: Medical Research Council | Value: £269,873

Cathelicidins as novel therapeutic antiviral agents in Rhinovirus infection

2015 - 2017
We have identified the human cathelicidn LL-37 as having potent direct antiviral activity against human rhinovirus (HRV), together with the capacity to alter death pathways in HRV-infected cells, as a...
Funder: Chief Scientists Office | Value: £163,152

IBD drug effect on autophagy (IDEA)

2017 - 2018
An investigation of commonly used IBD drugs on autophagy pathway activity and potential therapeutic benefit for treatment of paediatric IBD
Funder: Crohn's in Childhood Research Association | Value: £5,274

Development of a novel 3D culture system to understand bone:cartilage crosstalk in osteoarthritis

2018 - 2023
Osteoarthritis affects more than 9 million people in the UK and is the leading cause of disability. In osteoarthritis, joint tissues (cartilage and bone) undergo structural damage which ultimately pro...
Funder: Medical Research Scotland | Value: £130,430
10 results

The role of the human cathelicidin LL-37 in rhinovirus infection

2014 - 2019
The PhD project which I am undertaking is focused on understanding the role of cathelicidins during respiratory viral infections. Cathel...
Dr Filipa Henderson Sousa | Director of Studies: Prof Peter Barlow | Second Supervisor: Dr Craig Stevens

Nanocellulose-Host Defence Peptide (N-HDP) Conjugates as a Novel Therapeutic Antiviral Technology

2022 - date
Connan Masson | Director of Studies: Prof Peter Barlow | Second Supervisor: Dr Mark Dorris

The role of hypoxia and NOD2 in inflammatory bowel disease

2018 - 2021
Alice Arnold | Director of Studies: Dr Craig Stevens | Second Supervisor: Dr Jenny Fraser

Characterisation of autophagy modulation by thiopurines in inflammatory bowel disease

2018 - 2021
Connan Masson | Director of Studies: Dr Craig Stevens | Second Supervisor: Prof Peter Barlow

The effects of Inflammatory Bowel Disease drugs on the autophagy pathway

2014 - 2019
Inflammatory Bowel Disease (IBD), which includes Crohn’s disease (CD) and Ulcerative Colitis, is characterised by chronic inflam...
Kirsty Hooper | Director of Studies: Dr Craig Stevens | Second Supervisor: Prof Peter Barlow

Design and development of novel targeted anticancer drugs

2018 - date
Ewoma Akpughe | Director of Studies: Dr David Mincher | Second Supervisor: Agnes Turnbull

Player load monitoring and injury risk in elite Scottish Rugby Union

2017 - 2022
Cameron Paul | Director of Studies: Dr Tom Campbell | Second Supervisor: Dr Debbie Palmer

The role of the cytoskeleton in autophagy and Crohn's disease

2014 - 2020
Olga Biskou | Director of Studies: Dr Craig Stevens | Second Supervisor: Prof Peter Barlow

Development of a novel 3D culture system to understand bone: cartilage crosstalk in osteoarthritis

2018 - date
David Hughes | Director of Studies: Dr Craig Stevens | Second Supervisor: Dr Craig Stevens

Regulation of autophagy in cathelicidin/vitamin D signalling pathway

2014 - 2016
Sophie Shepherd | Director of Studies: Dr Craig Stevens | Second Supervisor: Prof Peter Barlow