Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
Journal Article
Kemp, S., Young, L., Ross, M., Prach, M., Hutchison, G. R., Malone, E., & Fraser, J. A. (2018)
Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-25854-2
Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to their impact on neutrop...
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters
Journal Article
Harvey, R. J., Harder, S., & Darlison, M. G. (1998)
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters. Technical tips online, 3(1), 150-152. https://doi.org/10.1016/S1366-2120%2808%2970126-0
Many laboratories still find the direct sequencing of large DNA templates (i.e. lambda, cosmid and bacteriophage P1 clones) problematic. To date, only limited amounts of seque...
Chromosomal localization of GABAA receptor subunit genes: relationship to human genetic disease
Journal Article
Buckle, V. J., Fujita, N., Ryder-Cook, A. S., Derry, J. M., Barnard, P. J., Lebo, R. V., …Barnard, E. A. (1989)
Chromosomal localization of GABAA receptor subunit genes: relationship to human genetic disease. Neuron, 3(5), 647-654. https://doi.org/10.1016/0896-6273%2889%2990275-4
Hybridization of {GABAA} receptor probes to human chromosomes in situ and to {DNA} from sorted human chromosomes has localized the genes encoding a β subunit and three isoform...
The molecular basis of muscular dystrophy in the mdx mouse: a point mutation
Journal Article
Sicinski, P., Geng, Y., Ryder-Cook, A., Barnard, E., Darlison, M., & Barnard, P. (1989)
The molecular basis of muscular dystrophy in the mdx mouse: a point mutation. Science, 244(4912), 1578-1580. https://doi.org/10.1126/science.2662404
The mdx mouse is an X-linked myopathic mutant, an animal model for human Duchenne muscular dystrophy. In both mouse and man the mutations lie within the dystrophin gene, but t...
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain
Journal Article
Albrecht, B. E., Breitenbach, U., Stühmer, T., Harvey, R. J., & Darlison, M. G. (1997)
In Situ Hybridization and Reverse Transcription-Polymerase Chain Reaction Studies on the Expression of the GABAc Receptor ρ1- and ρ2-subunit Genes in Avian and Rat Brain. European Journal of Neuroscience, 9(11), 2414-2422. https://doi.org/10.1111/j.1460-9568.1997.tb01658.x
The pharmacological properties of homo-oligomeric channels formed by the GABA type A receptor-like ρl and ρ2 polypeptides are very reminiscent of those of the GABA type C rece...
Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes
Journal Article
Albrecht, B. E., & Darlison, M. G. (1995)
Localization of the ρ1- and ρ2-subunit messenger RNAs in chick retina by in situ hybridization predicts the existence of γ-aminobutyric acid type C receptor subtypes. Neuroscience Letters, 189(3), 155-158. https://doi.org/10.1016/0304-3940%2895%2911479-g
We have amplified partial complementary {DNAs} for the chicken γ-aminobutyric acid type C (GABAC) receptor ρ1 and ρ2 subunits using the polymerase chain reaction. These nucleo...
Distinct regional expression of nicotinic acetylcholine receptor genes in chick brain
Journal Article
Morris, B. J., Hicks, A. A., Wisden, W., Darlison, M. G., Hunt, S. P., & Barnard, E. A. (1990)
Distinct regional expression of nicotinic acetylcholine receptor genes in chick brain. Molecular Brain Research, 7(4), 305-315. https://doi.org/10.1016/0169-328x%2890%2990081-n
Four genes (α2, α3, α4 and β2) have been reported as encoding subunits of the nicotinic acetylcholine receptor (nAChR) in chicken brain. The mRNAs transcribed from these genes...
The Structure and Expression of the GABAA Receptor as Deduced by Molecular Genetic Studies
Book Chapter
Darlison, M. G., Barnard, E. A., Bateson, A. N., Glencorse, T. A., Harvey, R. J., Hicks, A. A., …Wisden, W. (1989)
The Structure and Expression of the GABAA Receptor as Deduced by Molecular Genetic Studies. In Molecular Biology of Neuroreceptors and Ion Channels, 83-99. Springer Verlag. https://doi.org/10.1007/978-3-642-74155-5_7
It is well established that the inhibitory neurotransmitter γ-aminobutyric acid (GABA) mediates many of its effects by binding to the GABAA receptor, which is present on the m...
Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA
Journal Article
Fraser, S., Djamgoz, M., Usherwood, P., O'Brien, J., Darlison, M., & Barnard, E. (1990)
Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA. Molecular Brain Research, 8(4), 331-341. https://doi.org/10.1016/0169-328x%2890%2990047-h
Poly(A)+ messenger ribonucleic acid (mRNA) was extracted from leg muscles of the locust Schistocerca gregaria and injected into oocytes of Xenopus laevis. After 5–10 days incu...
Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain
Book Chapter
Squire, M. D., Moss, S. J., Lai, F. A., Darlison, M. G., Cockcroft, V. B., Barnard, E. A., …Ross, S. J. (1986)
Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain. In A. Maelicke (Ed.), Nicotinic Acetylcholine Receptor, 389-415. Springer Verlag. https://doi.org/10.1007/978-3-642-71649-2_30
While it has been completely established that the AChR** as isolated from the electroplaques of electric fish is composed of four different polypeptides, such that its subunit...