Weaker control of the electrical properties of cerebellar granule cells by tonically active GABAA receptors in the Ts65Dn mouse model of Down's syndrome.
Journal Article
Szemes, M., Davies, R. L., Garden, C. L. P., & Usowicz, M. M. (2013)
Weaker control of the electrical properties of cerebellar granule cells by tonically active GABAA receptors in the Ts65Dn mouse model of Down's syndrome. Molecular Brain, 6, 33
BACKGROUND
Down's syndrome (DS) is caused by triplication of all or part of human chromosome 21 and is characterized by a decrease in the overall size of the brain. One of th...
The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase
Journal Article
Boyd, G. W., Drew, M., Ward, S., Baird, M., Graham, S. M., & Connaboy, C. (2018)
The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase. Applied Physiology, Nutrition, and Metabolism, https://doi.org/10.1139/apnm-2018-0449
Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism...
FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression
Journal Article
Ikpegbu, E., Basta, L., Clements, D. N., Fleming, R., Vincent, T. L., Buttle, D. J., …Farquharson, C. (2018)
FGF-2 promotes osteocyte differentiation through increased E11/podoplanin expression. Journal of Cellular Physiology, 233(7), 5334-5347. https://doi.org/10.1002/jcp.26345
E11/podoplanin is critical in the early stages of osteoblast-to-osteocyte transitions (osteocytogenesis) however the upstream events which regulate E11 expression are unknown....
The effect of oxidative stress on macrophages and lung epithelial cells: The role of phosphodiesterases 1 and 4
Journal Article
Brown, D. M., HUTCHISON, L., DONALDSON, K., MACKENZIE, S., DICK, C., & STONE, V. (2007)
The effect of oxidative stress on macrophages and lung epithelial cells: The role of phosphodiesterases 1 and 4. Toxicology Letters, 168(1), 1-6. doi:10.1016/j.toxlet.2006.10.016
Reactive oxygen species (ROS) have been implicated in various pulmonary diseases by causing direct injury to lung epithelial cells. Signalling activity of cells through transc...
The local effects of ovarian diathermy in an ovine model of Polycystic Ovary Syndrome
Journal Article
Shen, W., Connolly, F., Rae, M. T., Butler, M., Klibanov, A. L., Sboros, V., …Duncan, W. C. (2014)
The local effects of ovarian diathermy in an ovine model of Polycystic Ovary Syndrome. PLOS ONE, 9, https://doi.org/10.1371/journal.pone.0111280
In order to develop a medical alternative to surgical ovarian diathermy (OD) in polycystic ovary syndrome (PCOS) more mechanistic information is required about OD. We therefor...
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
Journal Article
Houston, D. A., Staines, K. A., MacRae, V. E., & Farquharson, C. (2016)
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification. Journal of Visualized Experiments, 118(118), https://doi.org/10.3791/54978
The fundamental process of endochondral ossification is under tight regulation in the healthy individual so as to prevent disturbed development and/or longitudinal bone growth...
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity
Journal Article
Poulet, B., Liu, K., Plumb, D., Vo, P., Shah, M., Staines, K., …Bou-Gharios, G. (2016)
Overexpression of TIMP-3 in Chondrocytes Produces Transient Reduction in Growth Plate Length but Permanently Reduces Adult Bone Quality and Quantity. PLOS ONE, 11(12), https://doi.org/10.1371/journal.pone.0167971
Bone development and length relies on the growth plate formation, which is dependent on degradative enzymes such as MMPs. Indeed, deletion of specific members of this enzyme f...
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures
Journal Article
Houston, D. A., Myers, K., MacRae, V. E., Staines, K. A., & Farquharson, C. (2016)
The Expression of PHOSPHO1, nSMase2 and TNAP is Coordinately Regulated by Continuous PTH Exposure in Mineralising Osteoblast Cultures. Calcified Tissue International, 99(5), 510-524. https://doi.org/10.1007/s00223-016-0176-9
Sustained exposure to high levels of parathyroid hormone (PTH), as observed in hyperparathyroidism, are catabolic to bone. The increase in the RANKL/OPG ratio in response to c...
miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION
Journal Article
Mackenzie, N. C. W., Staines, K. A., Zhu, D., Genever, P., & MacRae, V. E. (2014)
miRNA-221 and miRNA-222 synergistically function to promote vascular calcification: MIR221/222 PROMOTE VASCULAR CALCIFICATION. Cell Biochemistry and Function, 32(2), 209-216. https://doi.org/10.1002/cbf.3005
Vascular calcification shares many similarities with skeletal mineralisation and involves the phenotypic trans-differentiation of vascular smooth muscle cells (VSMCs) to osteo...
Depletion of Drad21/Scc1 in Drosophila Cells Leads to Instability of the Cohesin Complex and Disruption of Mitotic Progression
Journal Article
Vass, S., Cotterill, S., Valdeolmillos, A. M., Barbero, J. L., Lin, E., Warren, W. D., & Heck, M. M. (2003)
Depletion of Drad21/Scc1 in Drosophila Cells Leads to Instability of the Cohesin Complex and Disruption of Mitotic Progression. Current Biology, 13(3), 208-218. doi:10.1016/S0960-9822(03)00047-2
Background: The coordination of cell cycle events is necessary to ensure the proper duplication and dissemination of the genome. In this study, we examine the consequences of ...