Drosophila melanogasterGlutamate-Cysteine Ligase Activity Is Regulated by a Modifier Subunit with a Mechanism of Action Similar to That of the Mammalian Form
Journal Article
Fraser, J. A., Saunders, R. D. C., & McLellan, L. I. (2002)
Drosophila melanogasterGlutamate-Cysteine Ligase Activity Is Regulated by a Modifier Subunit with a Mechanism of Action Similar to That of the Mammalian Form. Journal of Biological Chemistry, 277(2), 1158-1165. https://doi.org/10.1074/jbc.m106683200
Glutamate-cysteine ligase (GCL) plays an important role in regulating glutathione homeostasis. In mammals, it comprises a catalytic (GCLC) and modifier (GCLM) subunit. The exi...
The surface lipid of parasitic nematodes: Organization, and modifications during transition to the mammalian host environment
Journal Article
Proudfoot, L., Kusel, J. R., Smith, H. V., Harnett, W., Worms, M. J., & Kennedy, M. W. (1990)
The surface lipid of parasitic nematodes: Organization, and modifications during transition to the mammalian host environment. Acta tropica, 47(5-6), 323-330. https://doi.org/10.1016/0001-706x%2890%2990033-v
The biophysical properties of the surface lipid of a range of nematode species and their developmental stages were examined, using fluorescent lipid probes and fluorescence re...
In vivo in uterus non-invasive methods to predict placental insufficiency and adverse pregnancy outcomes in antiphospholipid syndrome
Conference Proceeding
Girardi, G., Fraser, J., Bertolaccini, M. L., Lennen, R., Vontell, R., & Hutchison, G. (2014)
In vivo in uterus non-invasive methods to predict placental insufficiency and adverse pregnancy outcomes in antiphospholipid syndrome. https://doi.org/10.1016/j.placenta.2014.06.184
Objectives: To use MRI-based methods to non invasively detect placental insufficiency and predict fetal outcomes in obstetric antiphospholipid syndrome (APS)
Methods and resul...
Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
Journal Article
Kemp, S., Young, L., Ross, M., Prach, M., Hutchison, G. R., Malone, E., & Fraser, J. A. (2018)
Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-25854-2
Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to their impact on neutrop...
The Isolation of Genomic DNA from Invertebrates
Book Chapter
Vreugdenhil, E., & Darlison, M. G. (1992)
The Isolation of Genomic DNA from Invertebrates. In A. Longstaff, & P. Revest (Eds.), Protocols in Molecular Neurobiology, 15-24. Springer Verlag. https://doi.org/10.1385/0-89603-199-3%3A15
Generally, genomic DNA is used either for the construction of genomic libraries or for Southern blot analysis. For several reasons, it is frequently preferable in the field of...
Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5
Journal Article
Sander, T., Hildmann, T., Janz, D., Wienker, T. F., Bianchi, A., Bauer, G., …Darlison, M. G. (1996)
Exclusion of linkage between idiopathic generalized epilepsies and the GABAA receptor α1 and γ2 subunit gene cluster on chromosome 5. Epilepsy Research, 23(3), 235-244. https://doi.org/10.1016/0920-1211%2895%2900098-4
Hereditary factors play a major role in the etiology of idiopathic generalized epilepsies (IGEs). The pivotal function of ionotropic γ-aminobutyric acid type A receptors (GABR...
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters
Journal Article
Harvey, R. J., Harder, S., & Darlison, M. G. (1998)
Reliable and accurate sequencing of lambda, cosmid and P1 DNAs using modified dye terminator reaction parameters. Technical tips online, 3(1), 150-152. https://doi.org/10.1016/S1366-2120%2808%2970126-0
Many laboratories still find the direct sequencing of large DNA templates (i.e. lambda, cosmid and bacteriophage P1 clones) problematic. To date, only limited amounts of seque...
Genetics of oxidative phosphorylation: Allelism studies of mitochondrial loci in the PHO1?OLI2 region of the genome
Journal Article
Darlison, M. G., & Lancashire, W. E. (1980)
Genetics of oxidative phosphorylation: Allelism studies of mitochondrial loci in the PHO1?OLI2 region of the genome. Molecular & general genetics : MGG, 180(1), 227-229. https://doi.org/10.1007/bf00267374
In a search for new aerobic-growth deficiency mutations affecting mitochondrial energy-conservation two mit− mutations, namely pho-8 and pho-9, have been isolated.
The two mut...
Chromosomal localization of GABAA receptor subunit genes: relationship to human genetic disease
Journal Article
Buckle, V. J., Fujita, N., Ryder-Cook, A. S., Derry, J. M., Barnard, P. J., Lebo, R. V., …Barnard, E. A. (1989)
Chromosomal localization of GABAA receptor subunit genes: relationship to human genetic disease. Neuron, 3(5), 647-654. https://doi.org/10.1016/0896-6273%2889%2990275-4
Hybridization of {GABAA} receptor probes to human chromosomes in situ and to {DNA} from sorted human chromosomes has localized the genes encoding a β subunit and three isoform...
The molecular basis of muscular dystrophy in the mdx mouse: a point mutation
Journal Article
Sicinski, P., Geng, Y., Ryder-Cook, A., Barnard, E., Darlison, M., & Barnard, P. (1989)
The molecular basis of muscular dystrophy in the mdx mouse: a point mutation. Science, 244(4912), 1578-1580. https://doi.org/10.1126/science.2662404
The mdx mouse is an X-linked myopathic mutant, an animal model for human Duchenne muscular dystrophy. In both mouse and man the mutations lie within the dystrophin gene, but t...