The Isolation of Genomic DNA from Invertebrates
Book Chapter
Vreugdenhil, E., & Darlison, M. G. (1992)
The Isolation of Genomic DNA from Invertebrates. In A. Longstaff, & P. Revest (Eds.), Protocols in Molecular Neurobiology, 15-24. Springer Verlag. https://doi.org/10.1385/0-89603-199-3%3A15
Generally, genomic DNA is used either for the construction of genomic libraries or for Southern blot analysis. For several reasons, it is frequently preferable in the field of...
Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms
Journal Article
Proudfoot, L., Kusel, J. R., Smith, H. V., Harnett, W., Worms, M. J., & Kennedy, M. W. (1993)
Rapid changes in the surface of parasitic nematodes during transition from pre- to post-parasitic forms. Parasitology, 107(01), 107. doi:10.1017/s0031182000079464
All mammalian-parasitic stages of a range of nematode species investigated (Brugia pahangi, Acanthocheilonema viteae, Strongyloides ratti, Nippostrongylus brasiliensis, Trichi...
Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major
Journal Article
Proudfoot, L., Schneider, P., Ferguson, M. A. J., & McConville, M. J. (1995)
Biosynthesis of the glycolipid anchor of lipophosphoglycan and the structurally related glycoinositolphospholipids from Leishmania major. The biochemical journal, 308(1), 45-55. https://doi.org/10.1042/bj3080045
The major macromolecule on the surface of the protozoan parasite Leishmania major is a lipophosphoglycan (LPG) which contains a glycosylphosphatidylinositol glycolipid anchor....
How dirty is your QWERTY? The risk of healthcare pathogen transmission from computer keyboards
Journal Article
Ledwoch, K., Dancer, S., Otter, J., Kerr, K., Roposte, D., & Maillard, J. (2021)
How dirty is your QWERTY? The risk of healthcare pathogen transmission from computer keyboards. Journal of Hospital Infection, 112, 31-36. https://doi.org/10.1016/j.jhin.2021.02.021
Introduction
Healthcare environmental surfaces may be contaminated with micro-organisms that cause healthcare-associated infections (HCAIs). Special attention is paid to near-...
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique
Journal Article
Lasham, A., & Darlison, M. G. (1993)
Direct sequencing of lambda DNA from crude lysates using an improved linear amplification technique. Molecular and Cellular Probes, 7(1), 67-73. doi:10.1006/mcpr.1993.1009
We described an improved method for directly sequencing lambda (λ) DNA that has been isolated from either crude cleared lysates or plate lysates. This protocol does not requir...
Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA
Journal Article
Fraser, S., Djamgoz, M., Usherwood, P., O'Brien, J., Darlison, M., & Barnard, E. (1990)
Amino acid receptors from insect muscle: electrophysiological characterization in Xenopus oocytes following expression by injection of mRNA. Molecular Brain Research, 8(4), 331-341. https://doi.org/10.1016/0169-328x%2890%2990047-h
Poly(A)+ messenger ribonucleic acid (mRNA) was extracted from leg muscles of the locust Schistocerca gregaria and injected into oocytes of Xenopus laevis. After 5–10 days incu...
Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain
Book Chapter
Squire, M. D., Moss, S. J., Lai, F. A., Darlison, M. G., Cockcroft, V. B., Barnard, E. A., …Ross, S. J. (1986)
Molecular Biology of Nicotinic Acetylcholine Receptors from Chicken Muscle and Brain. In A. Maelicke (Ed.), Nicotinic Acetylcholine Receptor, 389-415. Springer Verlag. https://doi.org/10.1007/978-3-642-71649-2_30
While it has been completely established that the AChR** as isolated from the electroplaques of electric fish is composed of four different polypeptides, such that its subunit...
Opioid receptors from a lower vertebrate (Catostomus commersoni): Sequence, pharmacology, coupling to a G-protein-gated inward-rectifying potassium channel (GIRK1), and evolution
Journal Article
Darlison, M. G., Greten, F. R., Harvey, R. J., Kreienkamp, H., Stühmer, T., Zwiers, H., …Richter, D. (1997)
Opioid receptors from a lower vertebrate (Catostomus commersoni): Sequence, pharmacology, coupling to a G-protein-gated inward-rectifying potassium channel (GIRK1), and evolution. Proceedings of the National Academy of Sciences, 94(15), 8214-8219
The molecular evolution of the opioid receptor family has been studied by isolating cDNAs that encode six distinct opioid receptor-like proteins from a lower vertebrate, the t...
Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA
Journal Article
Bhandal, N. S., Ramsey, R. L., Harvey, R. J., Darlison, M. G., & Usherwood, P. N. R. (1995)
Channel gating in the absence of agonist by a homooligomeric molluscan GABA receptor expressed inXenopus oocytes from a cloned cDNA. Invertebrate Neuroscience, 1(3), 267-272. https://doi.org/10.1007/bf02211028
We have previously described the isolation of a complementary DNA (cDNA) from the freshwater molluscLymnaea stagnalis encoding a polypeptide that exhibits ∼50{%} identity to t...
Cloning and functional pharmacology of two corticotropin-releasing factor receptors from a teleost fish
Journal Article
Pohl, S., Darlison, M. G., Clarke, W., Lederis, K., & Richter, D. (2001)
Cloning and functional pharmacology of two corticotropin-releasing factor receptors from a teleost fish. European Journal of Pharmacology, 430(2-3), 193-202. https://doi.org/10.1016/S0014-2999%2801%2901391-7
Although it is well established that fish possess corticotropin-releasing factor (CRF) and a CRF-like peptide, urotensin I, comparatively little is known about the pharmacolog...